Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay

R&D Systems | Catalog # SPCKB-PS-015537

Simple Plex Human Amyloid Beta aa1-42 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD06, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

2 hours 50 minutes

Sample Type & Volume Required Per Well*

Serum (40 µL), EDTA Plasma (40 µL), Heparin Plasma (40 µL), Cerebrospinal Fluid (40 µL)

Sensitivity

0.22 pg/mL

Assay Range

0.92-1409 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay Summary

Simple Plex Ultra-Sensitive Assay is for the detection of human Amyloid beta (amino acids 1-42) in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Amyloid beta Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural human Amyloid beta (aa1-42).

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Amyloid beta (aa1-42) assay.

Inter-Assay Precision (Precision between assays) Replicates of each Amyloid beta (aa1-42) control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Amyloid beta (aa1-42).

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 95 93
Mean (pg/mL) 12.1 534 11.5 557
Standard Deviation 0.696 23.9 1.26 59.9
CV% 5.8 4.5 10.9 10.8

Recovery for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay

Recovery was not necessary as human Amyloid beta (aa1-42) shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of Amyloid beta (aa1-42) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Linearity

Scientific Data Images for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay

Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Standard Curve

Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay. Assay range is 0.92-1409 pg/mL.
Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay

Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Endogenous Levels

Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

Additional Amyloid beta Products

Product Documents for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay

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Certificate of Analysis

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Product Specific Notices for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay

For research use only

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