SiRA 2
Tocris Bioscience | Catalog # 7544
Key Product Details
Description
Wavelength
Product Description
Key information: SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells.Application: confocal microscopy, super resolution microscopy (SRM) techniques, including super-resolution stimulated emission depletion (STED). Cell permeable.
Properties and Photophysical Data: SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Excitation and emission maxima (λ) are 649 nM and 662 nM, respectively; quantum yield = 0.98; extinction coefficient = 86,000 M-1cm-1; KD = 403 nM.
Reconstitute in 200 μL for a 0.5 mM stock solution. A typical working concentration for this product is 500 nM, see references provided for more details.
We recommend using 5mM Mg2+ (MgCl2 or MgSO4) in all buffers/media for maximum fluorescence intensity.
Licensing Information
Sold with kind permission of Prof. Andres JäschkeOptical Data
| Emission Color | Far Red |
| λabs | 649 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 86000 M-1cm-1 |
| Quantum Yield (φ) | 0.98 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
Spectra Viewer
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Product Specifications for SiRA 2
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 30.14 | 50 | |
| DMSO | 60.28 | 100 |
Preparing Stock Solutions for SiRA 2
The following data is based on the product molecular weight 602.81.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.66 mL | 8.29 mL | 16.59 mL |
| 5 mM | 0.33 mL | 1.66 mL | 3.32 mL |
| 10 mM | 0.17 mL | 0.83 mL | 1.66 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Wirth SiRA: A silicon rhodamine-binding aptamer for live-cell super-resolution RNA imaging. J.Am.Chem.Soc. 2019 PMID: 30986047
Product Documents for SiRA 2
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SiRA 2
For research use only
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