Sirtuin 1/SIRT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27205

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Amphibian, Bovine, Canine, Chicken, Chinese Hamster, Equine, Feline, Hamster, Primate, Rabbit

Cited:

Human, Mouse, Porcine, Hamster

Predicted:

Avian (100%), Chimpanzee (100%), Monkey (100%), Orangutan (100%), Rat (100%), Reptile (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Sirtuin 1/SIRT1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 400-450 from human SIRT1 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Frog, and White-cheeked Gibbon, and 89% homologous in Zebrafish. Use in Hamster reported in scientific literature (PMID:24585438).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 1/SIRT1 Antibody - BSA Free

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Sirtuin 1/SIRT1 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:50

Western Blot

2-4ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 94 kDa.

Reviewed Applications

Read 2 reviews rated 3.5 using NBP2-27205 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

SIRT1 is a nuclear protein and member of the sirtuin protein family of deacetylating proteins. SIRT1 is involved in regulating a number of cellular pathways including apoptosis and cell senescence, longevity and aging (1-3). Not surprisingly, SIRT1 targets to forkhead (FOXO) transcription factors. SIRT1 physically binds to the p65/RelA protein in the NF-kB complex and inhibits the transcriptional activity by deacetylating the p65 component (4). SIRT1 anti-inflammatory responses have also been demonstrated using resveratrol, a potent activator of SIRT1, which has been shown to potentiate the SIRT1-mediated anti-inflammatory response (5). Detailed involvement of SIRT1 and NF-kB, and the control and interconnection of the Innate Immune and inflammatory response and aging, is reviewed by Salminen et al (6).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Sirtuin 1/SIRT1 Antibody - BSA Free

Customer Reviews for Sirtuin 1/SIRT1 Antibody - BSA Free (2)

3.5 out of 5
2 Customer Ratings
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  • Sirtuin 1/SIRT1 Antibody
    Name: Francesca Pacifici
    Application: Western Blot
    Sample Tested: Mouse skeletal muscle homogenate
    Species: Mouse
    Verified Customer | Posted 09/26/2017
    Western Blot analysis was performed on mouse skeletal muscle proteins separated by 4-12% SDS-PAGE, transferred on nitrocellulose membrane, blocked 1h at room temperature with 5% non-fat milk, and probed with anti SIRT1 [NBP2-27205] 1:200.
    Western Blot analysis was performed on mouse skeletal muscle proteins separated by 4-12% SDS-PAGE, transferred on nitrocellulose membrane, blocked 1h at room temperature with 5% non-fat milk, and probed with anti SIRT1 [NBP2-27205] 1:200.
    Sirtuin 1/SIRT1 Antibody - BSA Free NBP2-27205
  • Sirtuin 1/SIRT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Caco-2 cell line
    Species: Human
    Verified Customer | Posted 05/04/2017
    The amount of galloflavine 0, 12.5 uM, 25 uM, 50 uM, 100 uM ja 200 uM in Caco2 cells in the experiment of 24 h.
    Sirtuin 1/SIRT1 Antibody - BSA Free NBP2-27205

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