SMC3 Antibody (8W9R7)

Novus Biologicals | Catalog # NBP3-15798

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8W9R7 expressed in HEK293
View all SMC3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1118-1217 of human SMC3 (NP_005436.1). GQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC3 Antibody (8W9R7)

Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798]

Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798]

Western Blot: SMC3 Antibody (8W9R7) [NBP3-15798] - Western blot analysis of extracts of various cell lines, using SMC3 Rabbit mAb (NBP3-15798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of C6 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of U2OS using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/ Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunocytochemistry/Immunofluorescence: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunofluorescence analysis of NIH/3T3 using SMC3 antibody (NBP3-15798) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798]

Immunoprecipitation: SMC3 Antibody (8W9R7) [NBP3-15798] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug SMC3 antibody (NBP3-15798). Western blot was performed from the immunoprecipitate using SMC3 antibody (NBP3-15798) at a dilition of 1:1000.

Applications for SMC3 Antibody (8W9R7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMC3

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Alternate Names

Bamacan, BAMSMC protein 3, Basement membrane-associated chondroitin proteoglycan, BMH, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), Chromosome-associated polypeptide, CSPG6structural maintenance of chromosomes protein 3, hCAP, HCAPbamacan, SMC-3, SMC3L1CDLS3, structural maintenance of chromosomes 3

Gene Symbol

SMC3

Additional SMC3 Products

Product Documents for SMC3 Antibody (8W9R7)

Certificate of Analysis

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Product Specific Notices for SMC3 Antibody (8W9R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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