SMC3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88715

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMC3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC3 Antibody - BSA Free

Western Blot: SMC3 Antibody [NBP1-88715]

Western Blot: SMC3 Antibody [NBP1-88715]

Western Blot: SMC3 Antibody [NBP1-88715] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG
Immunohistochemistry: SMC3 Antibody [NBP1-88715]

Immunohistochemistry: SMC3 Antibody [NBP1-88715]

Immunohistochemistry: SMC3 Antibody [NBP1-88715] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Simple Western: SMC3 Antibody [NBP1-88715]

Simple Western: SMC3 Antibody [NBP1-88715]

Simple Western: SMC3 Antibody [NBP1-88715] - Simple Western lane view shows a specific band for SMC3 in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.
Simple Western: SMC3 Antibody [NBP1-88715]

Simple Western: SMC3 Antibody [NBP1-88715]

Simple Western: SMC3 Antibody [NBP1-88715] - Electropherogram image of the corresponding Simple Western lane view. SMC3 antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.
SMC3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SMC3 Antibody - BSA Free [NBP1-88715]

Chromatin Immunoprecipitation-exo-Seq: SMC3 Antibody - BSA Free [NBP1-88715]

ChIP-Exo-Seq composite graph for Anti-SMC3 (NBP1-88715) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SMC3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-10µg per reaction

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251 MG lysates, separated by Size, antibody dilution of 1:25

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMC3

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Alternate Names

Bamacan, BAMSMC protein 3, Basement membrane-associated chondroitin proteoglycan, BMH, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), Chromosome-associated polypeptide, CSPG6structural maintenance of chromosomes protein 3, hCAP, HCAPbamacan, SMC-3, SMC3L1CDLS3, structural maintenance of chromosomes 3

Gene Symbol

SMC3

Additional SMC3 Products

Product Documents for SMC3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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