SMC4 Antibody (9Z8S3)

Novus Biologicals | Catalog # NBP3-16219

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Z8S3 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1189-1288 of human SMC4 (Q9NTJ3). FNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRLIGIYKTYNITKSVAVNPKEIASKGLC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC4 Antibody (9Z8S3)

Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219]

Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219]

Western Blot: SMC4 Antibody (9Z8S3) [NBP3-16219] - Western blot analysis of extracts of various cell lines, using SMC4 Rabbit mAb (NBP3-16219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219]

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219]

Immunocytochemistry/Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of U-2 OS cells using SMC4 Rabbit mAb (NBP3-16219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
SMC4 Antibody (9Z8S3)

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] -

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of NIH/3T3 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
SMC4 Antibody (9Z8S3)

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] -

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody (9Z8S3) [NBP3-16219] - Immunofluorescence analysis of C6 cells using SMC4 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for SMC4 Antibody (9Z8S3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMC4

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Alternate Names

CAP-C, CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast), Chromosome-associated polypeptide C, hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes 4, structural maintenance of chromosomes protein 4, XCAP-C homolog

Gene Symbol

SMC4

Additional SMC4 Products

Product Documents for SMC4 Antibody (9Z8S3)

Certificate of Analysis

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Product Specific Notices for SMC4 Antibody (9Z8S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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