SMN Antibody (4B7) - Azide Free

Novus Biologicals | Catalog # NBP3-11944

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 4B7

Format

Azide Free
View all SMN Primary Antibodies »

Product Specifications

Immunogen

Recombinant human his-tagged SMN protein expressed in and purified from E. coli. The epitope recognized by the 4B7 clone binds to the region encoded by exon 1. (UniProt: Q16637)

Specificity

Specific for endogenous levels of the ~35 kDa survival motor neuron protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMN Antibody (4B7) - Azide Free

Western Blot: SMN Antibody (4B7) [NBP3-11944]

Western Blot: SMN Antibody (4B7) [NBP3-11944]

Western Blot: SMN Antibody (4B7) [NBP3-11944] - Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.

Applications for SMN Antibody (4B7) - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SMN

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Alternate Names

Gemin-1, Kugelberg-Welander disease), SMNC, survival motor neuron protein, survival of motor neuron 1, telomeric

Gene Symbol

SMN1

Additional SMN Products

Product Documents for SMN Antibody (4B7) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SMN Antibody (4B7) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SMN Antibody (4B7) - Azide Free

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  • Q: Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.

    A: We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

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