SMN Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32021

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMN Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMN. The exact sequence is proprietary.

Reactivity Notes

Mouse (86%), Rat (84%), Pig (87%).

Localization

Cytoplasm, Nucleus, gem

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMN Antibody - BSA Free

Western Blot: SMN Antibody [NBP1-32021]

Western Blot: SMN Antibody [NBP1-32021]

Western Blot: SMN Antibody [NBP1-32021] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SMN1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SMN Antibody [NBP1-32021]

Immunocytochemistry/ Immunofluorescence: SMN Antibody [NBP1-32021]

Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP1-32021] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Applications for SMN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 40% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMN

Gemin 1 (SMN) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Alternate Names

Gemin-1, Kugelberg-Welander disease), SMNC, survival motor neuron protein, survival of motor neuron 1, telomeric

Gene Symbol

SMN1

Additional SMN Products

Product Documents for SMN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SMN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SMN Antibody - BSA Free

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  • Q: Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.

    A: We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

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