Q: Our customer needs SMUG1 protein for ASSAY ACTIVITY ? Does this product id # NBP2-22806 a good choice for that ??

A: Unfortunately, our SMUG1 Protein NBP2-22806 has not yet been tested for activity. We have only tested it for use in SDS PAGE gels.

Recombinant Human SMUG1 His Protein

Novus Biologicals | Catalog # NBP2-22806

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all SMUG1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-270 of Human SMUG1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

32.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SMUG1 His Protein

SDS-PAGE: Recombinant Human SMUG1 His Protein [NBP2-22806]

SDS-Page: Recombinant Human SMUG1 Protein [NBP2-22806]

Formulation, Preparation, and Storage

NBP2-22806

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 30% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMUG1

Single-strand-selective monofunctional uracil-DNA glycosylase, also known as SMUG1, is a enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. This protein acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. Recombinant human SMUG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Single-strand Selective Monofunctional Uracil DNA Glycosylase 1

Alternate Names

EC 3.2.2, EC 3.2.2.-, FDG, HMUDG, MGC104370, single-strand selective monofunctional uracil DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase 1, UNG3

Gene Symbol

SMUG1

Additional SMUG1 Products

Product Documents for Recombinant Human SMUG1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human SMUG1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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