Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

Novus Biologicals | Catalog # NBP2-59894

Novus Biologicals
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Key Product Details

Source

Baculovirus

Tag

His

Applications

Enzyme Activity, SDS-PAGE
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Product Specifications

Description

A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 63-303 of Human Sodium Potassium ATPase Beta 1

Source:Baculovirus

Amino Acid Sequence: ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 3,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

SDS-PAGE: Recombinant Human Sodium Potassium ATPase Beta 1 His Protein [NBP2-59894]

SDS-PAGE: Recombinant Human Sodium Potassium ATPase Beta 1 His Protein [NBP2-59894]

SDS-Page: Recombinant Human Sodium Potassium ATPase Beta 1 Protein [NBP2-59894] - 15% SDS-PAGE

Formulation, Preparation, and Storage

NBP2-59894
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Beta 1

Sodium Potassium ATPase Beta 1 (ATP1B1) is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. Na/K ATPase pumps consist of alpha/beta heterodimers. Beta 1 is the glycosylated, non-catalytic component of the active enzyme responsible for the formation of these alpha/beta heterodimers. Through this assembly, the beta polypeptide regulates the number of sodium pumps transported to the plasma membrane.

Long Name

Sodium/potassium-transporting ATPase subunit beta-1

Alternate Names

ATP1B, ATP1B1

Gene Symbol

ATP1B1

Additional Sodium Potassium ATPase Beta 1 Products

Product Documents for Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Sodium Potassium ATPase Beta 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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