Soluble Liver/Pancreas Antigen Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94530

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Soluble Liver/Pancreas Antigen Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human SEPSECS (NP_058651.3). STTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Soluble Liver/Pancreas Antigen Antibody - BSA Free

Western Blot: Soluble Liver/Pancreas Antigen AntibodyBSA Free [NBP2-94530]

Western Blot: Soluble Liver/Pancreas Antigen AntibodyBSA Free [NBP2-94530]

Western Blot: Soluble Liver/Pancreas Antigen Antibody [NBP2-94530] - Analysis of extracts of various cell lines, using Soluble Liver/Pancreas Antigen at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Soluble Liver/Pancreas Antigen Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Soluble Liver/Pancreas Antigen Antibody - BSA Free [NBP2-94530] -

Immunocytochemistry/ Immunofluorescence: Soluble Liver/Pancreas Antigen Antibody - BSA Free [NBP2-94530] - Immunofluorescence analysis of U2OS cells using Soluble Liver/Pancreas Antigen antibody (A7103). Blue: DAPI for nuclear staining.

Applications for Soluble Liver/Pancreas Antigen Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Soluble Liver/Pancreas Antigen

The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor (Palioura et al., 2009 (PubMed 19608919)).(supplied by OMIM)

Alternate Names

EC 2.9.1.2, EC 2.9.1.n1, Liver-pancreas antigen, LPDKFZp434B1417, MGC161491, O-phosphoseryl-tRNA(Sec) selenium transferase, Sec synthase, Selenocysteine synthase, Selenocysteinyl-tRNA(Sec) synthase, Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, SepSecS, Sep-tRNA:Sec-tRNA synthase, SLA/LP, SLA/LP autoantigen, SLA-p35, SLAUGA suppressor tRNA-associated protein, Soluble liver antigen, soluble liver antigen/liver pancreas antigen, tRNA(Ser/Sec)-associated antigenic protein, TRNP48

Gene Symbol

SEPSECS

Additional Soluble Liver/Pancreas Antigen Products

Product Documents for Soluble Liver/Pancreas Antigen Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Soluble Liver/Pancreas Antigen Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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