SP2 Antibody (HL3271)

Name: SP2 Antibody (HL3271)
Brand: Novus Biologicals
SKU: NBP3-48786
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48786

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3271

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Product Specifications

Immunogen

Recombinant fragment of human SP2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP2 Antibody (HL3271)

Western Blot: SP2 Antibody (HL3271) [NBP3-48786] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP2 antibody [HL3271] (NBP3-48786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: SP2 Antibody (HL3271) [NBP3-48786] -

SP2 antibody [HL3271] detects SP2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP2 stained by SP2 antibody [HL3271] (NBP3-48786) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for SP2 Antibody (HL3271)

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP2

The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3.

Alternate Names

KIAA0048, Sp2 transcription factor, transcription factor Sp2

Gene Symbol

SP2

Additional SP2 Products

Product Documents for SP2 Antibody (HL3271)

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Certificate of Analysis

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Product Specific Notices for SP2 Antibody (HL3271)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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