Spastin Antibody (RM346)
Novus Biologicals | Catalog # NBP2-77421
Recombinant Monoclonal Antibody
![Western Blot: Spastin Antibody (RM346) [NBP2-77421]](https://resources.rndsystems.com/images/products/Spastin-Antibody-RM346-Western-Blot-NBP2-77421-img0003.jpg "Western Blot: Spastin Antibody (RM346) [NBP2-77421]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM346 expressed in HEK293
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Product Specifications
Immunogen
A peptide corresponding to the N-terminus of human Spastin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Spastin Antibody (RM346)
Western Blot: Spastin Antibody (RM346) [NBP2-77421]
Western Blot: Spastin Antibody (RM346) [NBP2-77421] - Western Blot of lysate from HeLa cells, transfected with Control siRNA (1) or Spastin siRNA, using NBP2-77421.
Immunohistochemistry: Spastin Antibody (RM346) [NBP2-77421] -
Immunohistochemistry: Spastin Antibody (RM346) [NBP2-77421] - Immunohistochemical staining of formalin fixed and paraffin embedded human Brain tissue section using NBP2-77421.
Immunohistochemistry: Spastin Antibody (RM346) [NBP2-77421] -
Immunohistochemistry: Spastin Antibody (RM346) [NBP2-77421] - Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using NBP2-77421.Applications for Spastin Antibody (RM346)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Spastin
Alternate Names
ADPSPSpastic paraplegia 4 protein, FSP2EC 3.6.4.3, KIAA1083EC 3.6.1.3, spastic paraplegia 4 (autosomal dominant; spastin), spastin, SPG4
Gene Symbol
SPAST
Additional Spastin Products
Product Documents for Spastin Antibody (RM346)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Spastin Antibody (RM346)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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