SPRY2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-04099

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human Sprouty 2 using the numbering given in entry NP_005833.1 (GeneID 10253).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SPRY2 Antibody - BSA Free (NBP2-04099) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SPRY2 Antibody - BSA Free

Immunoprecipitation: SPRY2 Antibody [NBP2-04099]

Immunoprecipitation: SPRY2 Antibody [NBP2-04099]

Immunoprecipitation: SPRY2 Antibody [NBP2-04099] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Sprouty 2 antibody NBP2-04099 used for IP at 6 ug/mg lysate. Sprouty 2 was also immunoprecipitated by rabbit anti-Sprouty 2 antibody NBP2-04100, which recognizes a downstream epitope. For blotting immunoprecipitated Sprouty 2, NBP2-04100 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SPRY2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SPRY2

Sprouty 2 encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Long Name

Sprouty Homolog 2

Alternate Names

sprouty 2

Gene Symbol

SPRY2

Additional SPRY2 Products

Product Documents for SPRY2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPRY2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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