SSRP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84754

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SSRP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SSRP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-84754]

Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-84754]

Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-84754] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Simple Western: SSRP1 Antibody [NBP1-84754]

Simple Western: SSRP1 Antibody [NBP1-84754]

Simple Western: SSRP1 Antibody [NBP1-84754] - Electropherogram image of the corresponding Simple Western lane view. SSRP1 antibody was used at 1:25 dilution on Ntera-2 lysate(s) respectively.
SSRP1 Antibody - BSA Free Western Blot: SSRP1 Antibody - BSA Free [NBP1-84754]

Western Blot: SSRP1 Antibody - BSA Free [NBP1-84754]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2

Applications for SSRP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Ntera-2 lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 112 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SSRP1

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Alternate Names

Chromatin-specific transcription elongation factor 80 kDa subunit, cisplatin-DNA SSRP, facilitates chromatin remodeling 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT, FACT complex subunit SSRP1, FACT80FACT 80 kDa subunit, FACTp80, high mobility group box, hSSRP1, Recombination signal sequence recognition protein 1, structure specific recognition protein 1, Structure-specific recognition protein 1, T160

Gene Symbol

SSRP1

Additional SSRP1 Products

Product Documents for SSRP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SSRP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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