Synaptojanin 1 Antibody (5H1) - Azide Free

Novus Biologicals | Catalog # NBP1-47559

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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H1

Format

Azide Free
View all Synaptojanin 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein from the C-terminal region of rat synaptojanin 1. Accession # Q62910

Reactivity Notes

Tested in Rat.

Specificity

Specific for endogenous levels of the ~145 kDa synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptojanin 1 Antibody (5H1) - Azide Free

Western Blot: Synaptojanin 1 Antibody (5H1) [NBP1-47559]

Western Blot: Synaptojanin 1 Antibody (5H1) [NBP1-47559]

Western Blot: Synaptojanin 1 Antibody (5H1) [NBP1-47559] - Rat Hippocampal Lysate showing specific immunolabeling of the ~145k Synaptojanin protein.

Applications for Synaptojanin 1 Antibody (5H1) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synaptojanin 1

Synaptojanin-1 is a phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) that has a role in clathrin-coated pit dynamics Perera et al. (2006) [PubMed 17158794].[supplied by OMIM]

Alternate Names

EC 3.1.3, EC 3.1.3.36, KIAA0910, polyphosphoinositide phosphatase, 10synaptojanin-1, Synaptic inositol-14,5-trisphosphate 5-phosphatase 1, synaptojanin 1

Entrez Gene IDs

85238 (Rat)

Gene Symbol

SYNJ1

UniProt

Q62910 (Rat)

Additional Synaptojanin 1 Products

Product Documents for Synaptojanin 1 Antibody (5H1) - Azide Free

Certificate of Analysis

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Product Specific Notices for Synaptojanin 1 Antibody (5H1) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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