SynGAP Antibody (HL2292) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25707

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2292 expressed in HEK293

Format

Azide and BSA Free
View all SynGAP Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragmemt of human SynGAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SynGAP Antibody (HL2292) - Azide and BSA Free

SynGAP Antibody (HL2292) - Azide and BSA Free

Western Blot: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] -

Western Blot: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SYNGAP1 antibody [HL2292] (NBP3-25707) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SynGAP Antibody (HL2292) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] -

Immunocytochemistry/Immunofluorescence: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] - SYNGAP1 antibody [HL2292] detects SYNGAP1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SYNGAP1 stained by SYNGAP1 antibody [HL2292] (NBP3-25707) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for SynGAP Antibody (HL2292) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SynGAP

Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

Alternate Names

DKFZp761G1421, KIAA1938135kDa, MRD5, synaptic Ras GTPase activating protein 1, synaptic Ras GTPase activating protein 1 homolog (rat), Synaptic Ras-GAP 1

Gene Symbol

SYNGAP1

Additional SynGAP Products

Product Documents for SynGAP Antibody (HL2292) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SynGAP Antibody (HL2292) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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