SynGAP Antibody (JE57-79)

Western Blot: SynGAP Antibody (JE57-79) [NBP3-32988]

Western Blot: SynGAP Antibody (JE57-79) [NBP3-32988] - Western blot analysis of SynGAP on different lysates with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat hippocampus tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 148 kDa
Observed band size: 140/148 kDa

Exposure time: 1 minute 23 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32988) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988]

Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32988) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988]

Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling SynGAP with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32988, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).