TACS-XL In Situ Apoptosis Detection Kit - Basic
TACS-XL In Situ Apoptosis Detection Kit - Basic Summary
• Robust method for in situ apoptosis detection
• Uses BrdU-based methods
• Precise cellular labeling
• Signal enhancing methods results in high signal-to-noise ratio
Why Use the TACS-XL In Situ Apoptosis Detection Kit - Basic?
TACS•XL embodies a more robust approach for the in situ detection of apoptosis than other methods. The TACS•XL Kits are based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis. The incorporation of BrdU by TdT is more efficient than either biotinylated or digoxigenin labeled nucleotides used in other terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based assays. The detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. The combination of antibody specificity with the signal enhancing properties of biotin streptavidin results in precise cellular labeling and the highest signal-to-noise ratio observed in competitive testing.
The TACS•XL Basic Kit provides the reagents necessary for routine labeling, including two permeabilization reagents, labeling buffers and reagents, Strep-HRP and TACS-Nuclease reagents for generating positive controls with your own samples. It is ideal for researchers whose labs are equipped for standard immunohistochemical procedures involving horseradish-peroxidase want to adapt the assay for fluorescence detection. The kit doesn’t not contain any peroxydase-substrate or counterstain.
• Proteinase K
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• B-dNTP Mix
• anti-BrdU antibody
For research use only. Not for diagnositic use.
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