TACS-XL In Situ Apoptosis Detection Kit - Basic
TACS-XL In Situ Apoptosis Detection Kit - Basic Summary
• Robust method for in situ apoptosis detection
• Uses BrdU-based methods
• Precise cellular labeling
• Signal enhancing methods results in high signal-to-noise ratio
Why Use the TACS-XL In Situ Apoptosis Detection Kit - Basic?
TACS•XL embodies a more robust approach for the in situ detection of apoptosis than other methods. The TACS•XL Kits are based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis. The incorporation of BrdU by TdT is more efficient than either biotinylated or digoxigenin labeled nucleotides used in other terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based assays. The detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. The combination of antibody specificity with the signal enhancing properties of biotin streptavidin results in precise cellular labeling and the highest signal-to-noise ratio observed in competitive testing.
The TACS•XL Basic Kit provides the reagents necessary for routine labeling, including two permeabilization reagents, labeling buffers and reagents, Strep-HRP and TACS-Nuclease reagents for generating positive controls with your own samples. It is ideal for researchers whose labs are equipped for standard immunohistochemical procedures involving horseradish-peroxidase want to adapt the assay for fluorescence detection. The kit doesn’t not contain any peroxydase-substrate or counterstain.
• Proteinase K
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• B-dNTP Mix
• anti-BrdU antibody
For research use only. Not for diagnositic use.
Citations for TACS-XL In Situ Apoptosis Detection Kit - Basic
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The Role of MicroRNA-21 in Venous Neointimal Hyperplasia: Implications for Targeting miR-21 for VNH Treatment
Authors: S Kilari, C Cai, C Zhao, A Sharma, E Chernogubo, M Simeon, CC Wu, HL Song, L Maegdefess, S Misra
Mol. Ther., 2019;0(0):. 2019
Nuclear expression of beta-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells.
Authors: Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, Borges H, Wang J
Mol Cancer Res, 2013;11(3):207-18. 2013
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC
Int. J. Oncol., 2010;37(4):943-9. 2010
Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition.
Authors: Dempsey C, McCormick N, Croxford T, Seo Y, Grider A, Kelleher S
J Nutr, 0;142(4):655-60. 0
Characterization and quantification of proliferating cell patterns in endocapillary proliferation.
Authors: Wu Q, Tanaka H, Hirukawa T, Endoh M, Fukagawa M
Nephrol Dial Transplant, 0;27(8):3234-41. 0
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