TACS-XL In Situ Apoptosis Detection Kit - Basic

Catalog # Availability Size / Price Qty
4828-30-K
Product Details
Citations (6)
FAQs
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TACS-XL In Situ Apoptosis Detection Kit - Basic Summary

Provides the reagents necessary for routine labeling using TACS-based Assays.

Key Benefits

• Robust method for in situ apoptosis detection
• Uses BrdU-based methods
• Precise cellular labeling
• Signal enhancing methods results in high signal-to-noise ratio

Why Use the TACS-XL In Situ Apoptosis Detection Kit - Basic?

TACS•XL embodies a more robust approach for the in situ detection of apoptosis than other methods. The TACS•XL Kits are based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis. The incorporation of BrdU by TdT is more efficient than either biotinylated or digoxigenin labeled nucleotides used in other terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based assays. The detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. The combination of antibody specificity with the signal enhancing properties of biotin streptavidin results in precise cellular labeling and the highest signal-to-noise ratio observed in competitive testing.

The TACS•XL Basic Kit provides the reagents necessary for routine labeling, including two permeabilization reagents, labeling buffers and reagents, Strep-HRP and TACS-Nuclease reagents for generating positive controls with your own samples. It is ideal for researchers whose labs are equipped for standard immunohistochemical procedures involving horseradish-peroxidase want to adapt the assay for fluorescence detection. The kit doesn’t not contain any peroxydase-substrate or counterstain.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
•  B-dNTP Mix
• anti-BrdU antibody
• Strep-Diluent
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for TACS-XL In Situ Apoptosis Detection Kit - Basic

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. AZD4547 Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Inflammation: The Role of FGFR1 in Renal Tubular Epithelial Cells
    Authors: X Chen, X Zhang, J Xu, Y Zhao, J Bao, Z Zheng, J Han
    Drug Des Devel Ther, 2020;14(0):833-844.  2020
  2. The Role of MicroRNA-21 in Venous Neointimal Hyperplasia: Implications for Targeting miR-21 for VNH Treatment
    Authors: S Kilari, C Cai, C Zhao, A Sharma, E Chernogubo, M Simeon, CC Wu, HL Song, L Maegdefess, S Misra
    Mol. Ther., 2019;0(0):.  2019
  3. Nuclear expression of beta-catenin promotes RB stability and resistance to TNF-induced apoptosis in colon cancer cells.
    Authors: Han J, Soletti R, Sadarangani A, Sridevi P, Ramirez M, Eckmann L, Borges H, Wang J
    Mol Cancer Res, 2013;11(3):207-18.  2013
  4. TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC
    Int. J. Oncol., 2010;37(4):943-9.  2010
  5. Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition.
    Authors: Dempsey C, McCormick N, Croxford T, Seo Y, Grider A, Kelleher S
    J Nutr, 0;142(4):655-60.  0
  6. Characterization and quantification of proliferating cell patterns in endocapillary proliferation.
    Authors: Wu Q, Tanaka H, Hirukawa T, Endoh M, Fukagawa M
    Nephrol Dial Transplant, 0;27(8):3234-41.  0

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