TBIA

Key Product Details

Product Description

TBIA (trans-bis-isatoic anhydride) is a bifunctional SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) chemical cross-linking reagent. TBIA has two electrophilic isatoic anhydride moieties, both of which can react with the 2'-OH group in nucleotides adjacent to and distant from the primary RNA sequence. TBIA is used in SHAPE-JuMP (-juxtaposed merged pairs) a technique for exploring RNA tertiary structure proximity on large RNAs, and higher-order RNA-RNA interactions at nanometer resolution.

Product Specifications for TBIA

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for TBIA

The following data is based on the product molecular weight 248.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	20.15 mL	100.75 mL	201.49 mL
1 mM	4.03 mL	20.15 mL	40.30 mL
2 mM	2.01 mL	10.07 mL	20.15 mL
10 mM	0.40 mL	2.01 mL	4.03 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Christy Direct mapping of higher-order RNA interactions by SHAPE-JuMP. Biochemistry 2021 PMID: 34121404

Product Documents for TBIA