TcBuster™ Transposon pUC57 MCS AmpR

Gene delivery is a crucial step for a wide variety of genome engineering workflows, which have traditionally utilized viral vectors to achieve expression of exogenous DNA sequences. However, the generation of a virus is time consuming, inconsistent, and has limited cargo capacity. The TcBuster non-viral gene delivery system is a solution for stable integration of DNA cargo and addresses many of the limitations of viral vectors. It consists of the TcBuster-M Transposase mRNA and a compatible DNA TcBuster transposon, such as TcBuster Transposon pUC57 MCS AmpR. This product requires TcBuster Transposase mRNA to be functional.

This transposon includes a multiple cloning site for molecular cloning. For these applications, the plasmid contains an Ampicillin resistance gene. Molecular cloning will be required to insert DNA sequences of interest, such a promoter and genes of interest. After this step and when used in combination with TcBuster-M transposase mRNA, the DNA cargo will be inserted randomly into the host cell genome. This transposon serves as an ideal vector for custom DNA cargo for bioprocessing applications of the system.