TCR alpha/beta Antibody (TCR3) [FITC]

Novus Biologicals | Catalog # NBP1-28284

Novus Biologicals

Key Product Details

Species Reactivity

Avian, Chicken

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TCR3
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Product Specifications

Immunogen

CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells

Reactivity Notes

Chicken

Specificity

Chicken/Peacock/Guinea Fowl

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TCR alpha/beta Antibody (TCR3) [FITC]

Flow Cytometry: TCR alpha/beta Antibody (TCR3) [FITC] [NBP1-28284] - Chicken PBMC were double stained with mouse anti-chicken TCR a/b- FITC and mouse anti-chicken CD3-R-PE. Small lymphocytes were then gated and subjected to 2-color analysis on a Flow cytometer.

Applications for TCR alpha/beta Antibody (TCR3) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCR alpha/beta

MAb TCR3 precipitates a CD3-associated heterodimer of Mr 88-kDa (two bands of Mr 48-kDa and 40-kDa upon reduction) on chicken peripheral blood T cells. (1,2) Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 31-kDa from TCR3 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3, (1,2) arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. (3) The TCR3 MAb reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+ and CD4-CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are 'single positive, with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (ca. 80% CD4+ vs ca. 20% CD8+). (1,2)

Alternate Names

C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA

Gene Symbol

TRA

Additional TCR alpha/beta Products

Product Documents for TCR alpha/beta Antibody (TCR3) [FITC]

Certificate of Analysis

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Product Specific Notices for TCR alpha/beta Antibody (TCR3) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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