TDG Antibody (1142) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43595

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1142

Format

Azide and BSA Free
View all TDG Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TDG Antibody (1142) - Azide and BSA Free

Western Blot: TDG Antibody (1142) [NBP2-43595]

Western Blot: TDG Antibody (1142) [NBP2-43595]

Western Blot: TDG Antibody (1142) [NBP2-43595] - Analysis of Non-transfected (-) and TDG -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody diluted by 1:2500.
Immunocytochemistry/ Immunofluorescence: TDG Antibody (1142) [NBP2-43595]

Immunocytochemistry/ Immunofluorescence: TDG Antibody (1142) [NBP2-43595]

Immunocytochemistry/Immunofluorescence: TDG Antibody (1142) [NBP2-43595] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TDG protein stained by TDG antibody [1142] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Applications for TDG Antibody (1142) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TDG

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Long Name

Thymine DNA Glycosylase

Alternate Names

EC 3.2.2.29, thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Gene Symbol

TDG

Additional TDG Products

Product Documents for TDG Antibody (1142) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TDG Antibody (1142) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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