TdT Antibody (rDNTT/6909) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14198

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rDNTT/6909

Format

Azide and BSA Free
View all TdT Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-100) of human TdT protein (exact sequence is proprietary) (Uniprot: P04053)

Localization

Nucleus

Marker

Acute Lymphoblastic Leukemia Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13871).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TdT Antibody (rDNTT/6909) - Azide and BSA Free

TdT Antibody (rDNTT/6909) - Azide and BSA Free Immunohistochemistry-Paraffin: TdT Antibody (rDNTT/6909) - Azide and BSA Free [NBP3-14198] -

Immunohistochemistry-Paraffin: TdT Antibody (rDNTT/6909) - Azide and BSA Free [NBP3-14198] -

Immunohistochemistry-Paraffin: TdT Antibody (rDNTT/6909) - Azide and BSA Free [NBP3-14198] - Formalin-fixed, paraffin-embedded human thymus stained with TdT antibody (rDNTT/6909).

Applications for TdT Antibody (rDNTT/6909) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: use this antibody at 2-4ug/ml. For coating, order this antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TdT

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Long Name

Terminal Deoxynucleotidyl Transferase

Alternate Names

DNTT

Gene Symbol

DNTT

Additional TdT Products

Product Documents for TdT Antibody (rDNTT/6909) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TdT Antibody (rDNTT/6909) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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