TFAX 488, SE

Tocris Bioscience | Catalog # 6619

Green fluorescent dye; amine reactive
Tocris Bioscience
Discontinued Product
6619 has been discontinued. View all TFAX Dyes products.

Key Product Details

Description

Green fluorescent dye; amine reactive

Wavelength

Excitation = 495 nm, Emission = 515 nm
View all TFAX Dyes »

Product Description

TFAX 488, SE is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data

TFAX 488, SE Dye Spectra
Emission Color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488, AF 488, FITC, Cy2

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Product Specifications for TFAX 488, SE

Molecular Weight

643.41

Formula

C25H15N3O13S2Li2

Storage

Store at -20°C

Chemical Name

3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt

CAS Number

222164-96-7

PubChem ID

23543054

InChI Key

FYCGGEVYPRTFDS-UHFFFAOYSA-L

SMILES

[NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for TFAX 488, SE

Certificate of Analysis

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Product Specific Notices for TFAX 488, SE

For research use only

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