TFAX 488, SE

Catalog # Availability Size / Price Qty
6619/1
TFAX 488, SE | CAS No. 222164-96-7 | TFAX Dyes
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Description: Green fluorescent dye; amine reactive

Chemical Name: 3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt

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Biological Activity

Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data for TFAX 488, SE

Emission Color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488, AF 488, FITC, Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

M.Wt:
643.41
Formula:
C25H15N3O13S2Li2
Solubility:
Soluble in DMSO
Storage:
Store at -20°C
CAS No:
222164-96-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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