TFAX 488, SE
Chemical Name: 3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt
Biological ActivityTFAX 488, SE is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
Optical Data for TFAX 488, SE
|Extinction Coefficient (ε)||73,000 M-1cm-1|
|Quantum Yield (φ)||0.92|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|pH Sensitivity||pH insensitive pH 4-10|
|Application||flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED|
|Alternative For||Alexa Fluor® 488, AF 488, FITC, Cy2|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
Panchuk?Voloshina et al.
Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes.
Rossi et al.
J.Cell Sci., 2012;125:4405
Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation.
Iwata et al.
Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules.
Burnette et al.
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde et al.
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