TFAX 546, SE
Chemical Name: 6-[2-Carboxy-3,4,6-trichloro-5-[[2-[[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-2-oxoethyl]thio]phenyl]-1,2,3,4,8,9,10,11-octahydro-2,2,4,8,10,10-hexamethyl-12,14-disulfopyrano[3,2-g:5,6-g']diquinolin-13-ium triethylammonium salt
Biological ActivityAmine reactive yellow fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in microscopy. Excitation maximum = 554 nm; emission maximum = 570 nm; extinction coefficient = 112,000 M-1cm-1; quantum yield = 0.79.
Optical Data for TFAX 546, SE
|Extinction Coefficient (ε)||112000 M-1cm-1|
|Quantum Yield (φ)||0.79|
|Closest Laser line||532 nm|
|Reactive Group||NHS ester|
|pH Sensitivity||pH insensitive pH 4-10|
|Alternative For||TMR, Cyanine3, Alexa Fluor 546 SE, AF 546 SE|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
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Hone et al.
Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation.
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