Thioflavin T

Key Product Details

Product Description

Key information: Thioflavin T is a cell-permeable fluorescent amyloid stain.

Used for: staining of insoluble senile plaques of Aβ in brain tissues, monitoring the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro.

Application: fluorescence microscopy.

Properties and Photophysical Data: Thioflavin T is a cationic benzothiazole. It increases in fluorescence upon strong binding (K_i = 580 nM) to the stacked β sheets of amyloid fibrils. Excitation and emission maxima (λ) are 440 nm and 485 nm, respectively; quantum yield = 0.43; extinction coefficient = 31600 M^-1cm^-1.

This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data

Product Specifications for Thioflavin T

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for Thioflavin T

The following data is based on the product molecular weight 318.86.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.36 mL	156.81 mL	313.62 mL
0.5 mM	6.27 mL	31.36 mL	62.72 mL
1 mM	3.14 mL	15.68 mL	31.36 mL
5 mM	0.63 mL	3.14 mL	6.27 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Lee Fluorescence chemicals to detect insoluble and soluble amyloid-β aggregates. ACS Chem.Neurosci. 2019 PMID: 31009195
• Schindler Small, seeding-competent huntingtin fibrils are prominent aggregate species in brains of zQ175 Huntington's disease knock-in mice. Front.Neurosci. 2021 PMID: 34239412
• Doi RNA-binding protein TLS is a major nuclear aggregate-interacting protein in huntingtin exon 1 with expanded polyglutamine-expressing cells. J.Biol.Chem. 2008 PMID: 18167354

Product Documents for Thioflavin T