THP-1 TO901317 Treated / Untreated Cell Lysate

SDS-PAGE

THP whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).

Cell

TO901317 Treated / Untreated

THP-1 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then serum starved for 24 hours and treated with or without 1 uM TO901317 for 24 hours. They are then washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.

The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.

1.0 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.