Recombinant Human Thymosin beta 4 Protein

Novus Biologicals | Catalog # NBP2-35087

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

P62328

Applications

Bioactivity, SDS-PAGE
View all Thymosin beta 4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 43 amino acids corresponding to Thymosin beta 4 Source: E. coli

Uniprot ID: P62328

Amino Acid Sequence: SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Thymosin beta 4 as determined by LAL method.

Predicted Molecular Mass

4.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Thymosin beta 4 protein is fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Thymosin beta 4 Protein

SDS-PAGE: Recombinant Human Thymosin beta 4 Protein [NBP2-35087]

SDS-PAGE: Recombinant Human Thymosin beta 4 Protein [NBP2-35087]

SDS-Page: Human Thymosin beta 4 Protein [NBP2-35087]

Formulation, Preparation, and Storage

NBP2-35087
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thymosin beta 4

Thymosin Beta 4 is a naturally occurring peptide encoded by the TMSB4X gene located on Chr. X in humans. It is found in high concentrations in blood platelets, wound fluid and other tissues in the body. T beta-4 is a major actin regulating peptide and the primary function is to stimulate the productions of T cells, which plays important part of the immune system. The thymosin beta- 4 peptide, if used after a heart attack, might reactivate cardiac progenitor cells to repair damaged heart tissue.

Alternate Names

TB4X, THYB4, TMSB4, TMSB4X

Gene Symbol

TMSB4X

UniProt

P62328 (Human)

Additional Thymosin beta 4 Products

Product Documents for Recombinant Human Thymosin beta 4 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Thymosin beta 4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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