TIF1 alpha Antibody (23GB4235)

Novus Biologicals | Catalog # NBP3-51264

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4235
View all TIF1 alpha Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human TIF1 alpha (Uniprot: O15164)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIF1 alpha Antibody (23GB4235)

TIF1 alpha Antibody (23GB4235)

Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody (23GB4235)[NBP3-51264] -

Immunocytochemical staining of HepG2 cells with TIF1 alpha antibody (1:1,000). Nuclei were stained blue with DAPI; TIF1 alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
TIF1 alpha Antibody (23GB4235)

Flow Cytometry: TIF1 alpha Antibody (23GB4235)[NBP3-51264] -

Validation of TIF1 alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TIF1 alpha antibody (1:2,000) and analyzed using BD flow cytometer.
TIF1 alpha Antibody (23GB4235)

Flow Cytometry: TIF1 alpha Antibody (23GB4235)[NBP3-51264] -

Flow cytometric analysis of TIF1 alphaexpression in HepG2 cells using TIF1 alpha antibody (1:2,000). Green, isotype control; red, Tripartite motif containing 24.
TIF1 alpha Antibody (23GB4235)

Western Blot: TIF1 alpha Antibody (23GB4235)[NBP3-51264] -

Western blotting analysis using TIF1 alpha antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
TIF1 alpha Antibody (23GB4235)

Western Blot: TIF1 alpha Antibody (23GB4235)[NBP3-51264] -

Western blotting analysis using TIF1 alpha antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for TIF1 alpha Antibody (23GB4235)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIF1 alpha

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Gene Symbol

TRIM24

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody (23GB4235)

Certificate of Analysis

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Product Specific Notices for TIF1 alpha Antibody (23GB4235)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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