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Key Product Details
Species
Mouse
Applications
Western Blot
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Product Specifications
Application Notes
SF9 insect cells were transfected with a fusion protein
containing the 50 kD extracellular portion of mouse
TLR2 and a 30 kD human Fc region. Load 15 ul/lane
of a 12-well gel.
Specificity
Mouse TLR2 Transfected Cell Lysate
Type
Cell
Scientific Data Images for TLR2 Cell Lysate
Western Blot: TLR2 Cell Lysate [NBP2-30165]
Western Blot: TLR2 Cell Lysate [NBP2-30165] - 15 ul of mouse TLR2 transfected cell lysate tested with IMG-526 (A) and IMG-662 (B).Formulation, Preparation, and Storage
Formulation
1X Sample Buffer containing b-Mercaptoethanol
1 x SDS sample buffer:
60 mM Tris-Hcl (pH 6.8)
10 mM EDTA
2 % SDS
0.03% Bromophenol Blue
10% glycerol
0.3% b-mercaptoethanol
1 x SDS sample buffer:
60 mM Tris-Hcl (pH 6.8)
10 mM EDTA
2 % SDS
0.03% Bromophenol Blue
10% glycerol
0.3% b-mercaptoethanol
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TLR2
Long Name
Toll-like Receptor 2
Alternate Names
CD282
Gene Symbol
TLR2
Additional TLR2 Products
Product Documents for TLR2 Cell Lysate
Product Specific Notices for TLR2 Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars