TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-24536
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Key Product Details
Sample Type & Volume Required Per Well
serum, plasma, urine, animal tissue and cell samples
Sensitivity
9.22 U/L
Assay Range
9.22 - 100 U/L
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Product Specifications
Kit Type
Activity Assay Kit (Colorimetric)
Species
Non-species specific
Description
Detection instrument: Microplate reader (410 - 415 nm, optimum wavelength: 405 nm)
Assay time: 25 min
Assay time: 25 min
Precision
Intra-Assay Precision (Precision within an assay) %CV 2
Inter-Assay Precision (Precision between assays) %CV 2.3
Recovery for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
Recovery
Average recovery rate: 101%
Scientific Data Images for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] -
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] - Standard Reference Curve
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] -
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] - Assay ExampleKit Contents for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
- Microplate
- Plate Sealer
- Reagent 1: Buffer Solution
- Reagent 2: Substrate
- Reagent 3: Tartaric Acid Solution
- Reagent 4: Standard Substance
- Reagent 5: Chromogenic Agent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: TRACP/PAP/ACP5
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
Additional TRACP/PAP/ACP5 Products
Product Documents for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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