Tricyclic cytosine tC
Tocris Bioscience | Catalog # 7814
Key Product Details
Description
Wavelength
Product Description
Tricyclic cytosine tC is a fluorescent cytosine analog for imaging RNA in living cells. Enables the investigation of RNA synthesis, degradation, and trafficking at single-cell resolution when combined with confocal imaging. Excitation/emission maxima (λ) = 375/505 nm; extinction coefficient = 4000 M-1 cm-1; quantum yield = 0.2.Optical Data
| λabs | 375 nm |
| λem | 505 nm |
| Extinction Coefficient (ε) | 4000 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Application | Confocal Imaging |
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Product Specifications for Tricyclic cytosine tC
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 34.94 | 100 |
Preparing Stock Solutions for Tricyclic cytosine tC
The following data is based on the product molecular weight 349.36.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.31 mL | 28.62 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.72 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Stengel Incorporation of the fluorescent ribonucleotide analogue tCTP by T7 RNA polymerase. Anal.Chem. 2010 PMID: 20067253
- Sandin Fluorescent properties of DNA base analogue tC upon incorporation into DNA--negligible influence of neighbouring bases on fluorescence quantum yield. Nucleic Acids Res. 2005 PMID: 16147985
- Wang Live-cell RNA imaging with metabolically incorporated fluorescent nucleosides. J.Am.Chem.Soc. 2022 PMID: 35930766
Product Documents for Tricyclic cytosine tC
Certificate of Analysis
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Product Specific Notices for Tricyclic cytosine tC
For research use only
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