TRIM2 Antibody (OTI2B10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74606

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B10

Format

Azide and BSA Free
View all TRIM2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 1500-1600 of human SHPRH (NP_775105) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM2 Antibody (OTI2B10) - Azide and BSA Free

Western Blot: TRIM2 Antibody (OTI2B10)Azide and BSA Free [NBP2-74606]

Western Blot: TRIM2 Antibody (OTI2B10)Azide and BSA Free [NBP2-74606]

Western Blot: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2.
Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TRIM2 antibody, and then analyzed by flow cytometry.
Western Blot: TRIM2 Antibody (OTI2B10)Azide and BSA Free [NBP2-74606]

Western Blot: TRIM2 Antibody (OTI2B10)Azide and BSA Free [NBP2-74606]

Western Blot: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.
Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606] - Analysis of Hela cells, using anti-TRIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606]

Flow Cytometry: TRIM2 Antibody (OTI2B10) - Azide and BSA Free [NBP2-74606] - Analysis of Jurkat cells, using anti-TRIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for TRIM2 Antibody (OTI2B10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:1000-2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRIM2

TRIM2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Alternate Names

KIAA0517tripartite motif protein 2, RING finger protein 86, RNF86tripartite motif protein TRIM2, tripartite motif containing 2, tripartite motif-containing 2, tripartite motif-containing protein 2

Gene Symbol

TRIM2

Additional TRIM2 Products

Product Documents for TRIM2 Antibody (OTI2B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TRIM2 Antibody (OTI2B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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