TRIM9 Antibody (OTI2D6)

Novus Biologicals | Catalog # NBP2-03794

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D6
View all TRIM9 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 284-669 of human TRIM9 (NP_443210) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM9 Antibody (OTI2D6)

Western Blot: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Western Blot: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Western Blot: TRIM9 Antibody (OTI2D6) [NBP2-03794] - Analysis of extracts (10ug) from 2 different cell lines by using anti-TRIM9 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Immunocytochemistry/ Immunofluorescence: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Immunocytochemistry/Immunofluorescence: TRIM9 Antibody (OTI2D6) [NBP2-03794] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM9.
Flow Cytometry: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Flow Cytometry: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Flow Cytometry: TRIM9 Antibody (2D6) [NBP2-03794] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM9 antibody, and then analyzed by flow cytometry.
Western Blot: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Western Blot: TRIM9 Antibody (OTI2D6) [NBP2-03794]

Western Blot: TRIM9 Antibody (2D6) [NBP2-03794] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.

Applications for TRIM9 Antibody (OTI2D6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM9

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq].

Long Name

Tripartite Motif-containing 9

Alternate Names

KIAA0282, RNF91, SPRING

Entrez Gene IDs

114088 (Human)

Gene Symbol

TRIM9

Additional TRIM9 Products

Product Documents for TRIM9 Antibody (OTI2D6)

Certificate of Analysis

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Product Specific Notices for TRIM9 Antibody (OTI2D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Infectious Diseases

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Protocols

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