Recombinant Human Troponin I Type 1 (slow skeletal) His Protein

Novus Biologicals | Catalog # NBP2-22892

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-187 of Human Troponin I Type 1 (slow skeletal)

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

23.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Troponin I Type 1 (slow skeletal) His Protein

SDS-PAGE: Recombinant Human Troponin I Type 1 (slow skeletal) His Protein [NBP2-22892]

SDS-PAGE: Recombinant Human Troponin I Type 1 (slow skeletal) His Protein [NBP2-22892]

SDS-Page: Recombinant Human Troponin I Type 1 (slow skeletal) Protein [NBP2-22892]

Formulation, Preparation, and Storage

NBP2-22892
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I Type 1 (slow skeletal)

Troponin I, slow skeletal muscle, also known as TNNI1, belongs to the troponin I family. TNNI1 is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Recombinant human TNNI1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Gene Symbol

TNNI1

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Recombinant Human Troponin I Type 1 (slow skeletal) His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Troponin I Type 1 (slow skeletal) His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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