Tyrosine Phosphatase Substrate I, DADEY(PO3)LIPQQG

R&D Systems | Catalog # ES006

Substrate for PTP1B, TC-PTP, SHP-2, and Tyrosine Phosphatase
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Product Specifications

Source


Asp-Ala-Asp-Glu-Tyr(PO3)-Leu-Ile-Pro-Gln-Gln-Gly
Synthetic phosphopeptide corresponding to amino acids 988 - 998 of the mature EGF receptor (EGF R, Accession # AF288738).
Derived from an autophosphorylation site at Y992 of EGF R.

Purity

>95%, by HPLC.

Predicted Molecular Mass

1328.32 Da

Formulation, Preparation, and Storage

ES006
Formulation Lyophilized from a stock solution in distilled water.
Reconstitution Dissolves in water.
61.3 μL gives a stock solution of substrate at 10 mM concentration
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Lyophilized samples are stable for up to twelve months from date of receipt at -20° C to -70° C. Upon reconstitution, the substrate can be stored at 2° - 8° C for one month or at -20° to -70° C in a manual defrost freezer for three months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phosphatase Peptide Substrates

This phosphopeptide is a substrate for a variety of protein tyrosine phosphatases (PTPs), including PTP1B (R&D Systems, Catalog # 1366-PT), Yersinia PTP, and SHP-2. Enzymatic cleavage of this substrate liberates inorganic phosphate, which can be measured in dye binding assays, such as the malachite green method.

References

  1. Zhang, Z.Y. et al. (1993) Proc. Natl. Acad. Sci. USA 90:4446.
  2. Peters, G.H. et al. (2000) J. Biol. Chem. 275:18201.
  3. Sarmiento, M. et al. (1998) J. Biol. Chem. 273:26368.
  4. Sugimoto, S. et al. (1993 J. Biol. Chem. 268:22771.
  5. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.

Additional Phosphatase Peptide Substrates Products

Product Documents for Tyrosine Phosphatase Substrate I, DADEY(PO3)LIPQQG

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Product Specific Notices for Tyrosine Phosphatase Substrate I, DADEY(PO3)LIPQQG

For research use only

Citations for Tyrosine Phosphatase Substrate I, DADEY(PO3)LIPQQG

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