UBA52 Antibody - BSA Free
Novus Biologicals | Catalog # NB300-263
![Western Blot: UBA52 Antibody [NB300-263]](https://resources.rndsystems.com/images/products/UBA52-Antibody-Western-Blot-NB300-263-img0004.jpg "Western Blot: UBA52 Antibody [NB300-263]")
Key Product Details
Species Reactivity
Human, Mouse, Bovine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residues 1 and 50 of human Ubiquitin using the numbering given in entry P62988.(GeneID 7311).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UBA52 Antibody - BSA Free
Western Blot: UBA52 Antibody [NB300-263]
Western Blot: UBA52 Antibody [NB300-263] - Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified human ubiquitin was applied in decreasing amounts from 5 to 0.1 mcg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody NB 300-263 used at 0.4 mcg/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).![Immunohistochemistry: UBA52 Antibody [NB300-263]](https://resources.rndsystems.com/images/products/UBA52-Antibody-Immunohistochemistry-NB300-263-img0006.jpg "Immunohistochemistry: UBA52 Antibody [NB300-263]")
Immunohistochemistry: UBA52 Antibody [NB300-263]
Immunohistochemistry: UBA52 Antibody [NB300-263] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Ubiquitin used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for UBA52 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Western Blot
1:1000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UBA52
Alternate Names
CEP52ubiquitin carboxyl extension protein 52, HUBCEP52, MGC126879, MGC126881, MGC57125, RPL40, UBCEP2, ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40, ubiquitin-52 amino acid fusion protein, ubiquitin-60S ribosomal protein L40, ubiquitin-CEP52
Gene Symbol
UBA52
Additional UBA52 Products
Product Documents for UBA52 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UBA52 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for UBA52 Antibody - BSA Free
Powered by Bioz
Customer Reviews for UBA52 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review UBA52 Antibody - BSA Free and earn rewards!
Have you used UBA52 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...