UBE3B Antibody - BSA Free

Western Blot: UBE3B Antibody [NBP1-54950]

Western Blot: UBE3B Antibody [NBP1-54950] - 293T cells lysate, concentration 0.2-1 ug/ml.

Western Blot: UBE3B Antibody - BSA Free [NBP1-54950] -

VHL inhibits the UBE3B-mediated tumorigenic potential of breast cancer cells.A Protein levels of Flag-VHL and Myc-UBE3B in the stable MDA-MB-231 cell line expressing Flag-VHL or Myc-UBE3B. B The cell proliferation rate was measured in the indicated MDA-MB-231 cells (mean +/- SD, n = 3). ****P < 0.0001, by 2-way ANOVA Tukey’s multiple comparisons test. hrs, hours. C, D Colony formation was conducted in the indicated MDA-MB-231 cells, and representative images from three experiments were shown in (C). Colony numbers (D) were quantified (mean +/- SD, n = 3). ****P < 0.0001, by 1-way ANOVA Tukey’s multiple comparisons test. E, F Invasion assays were carried out in the indicated MDA-MB-231 cells, and representative images from three experiments were shown in (E). Invaded cell numbers were quantified in (F) (mean +/- SD, n = 3). ****P < 0.0001, by 1-way ANOVA Tukey’s multiple comparisons test. G Protein levels of VHL and UBE3B were detected in control, VHL-KD#1, UBE3B-KD, and VHL- and UBE3B-double knockdown (VHL-KD#1 + UBE3B-KD) MDA-MB-231 cells. H Proliferation rate of control, VHL-KD#1, UBE3B-KD, and VHL-KD#1+UBE3B-KD MDA-MB-231 cells (mean +/- SD, n = 3). ****P < 0.0001, by 2-way ANOVA Tukey’s multiple comparisons test. hrs, hours. I–L Colony formation (I, J) and invasion (K, L) assays were performed in control, VHL-KD#1, UBE3B-KD, and VHL-KD#1+UBE3B-KD MDA-MB-231 cells, and the representative images from three experiments were shown. Colony numbers (J) and invaded cell numbers (L) were quantified (mean +/- SD, n = 3). ****P < 0.0001, by 1-way ANOVA Tukey’s multiple comparisons test. Scale bars: 100 μm. EV, empty vector. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38914543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: UBE3B Antibody - BSA Free [NBP1-54950] -

VHL decreases UBE3B protein levels in breast cancer cells.A Protein levels of endogenous UBE3B were assessed in MDA-MB-231 and T47D cells stably expressing either wild-type (WT) or catalytically inactive (Y112H) Flag-VHL. B Protein levels of endogenous UBE3B in control or VHL-knockdown (VHL-KD) MDA-MB-231 and T47D cells. C–F The half-life of UBE3B protein in MDA-MB-231 (C, D) or T47D (E, F) cells expressing Flag-VHL. Cells were treated with 10 μg/mL CHX for the indicated time (mean +/- SD, n = 3). ****P < 0.0001, by 2-way ANOVA Tukey’s multiple comparisons test. G–J The half-life of UBE3B protein was analyzed in scrambled shRNA (SC) or VHL-KD MDA-MB-231 (G, H) or T47D (I, J) cells. Cells were treated with 10 μg/mL CHX for the indicated time (mean +/- SD, n = 3). ****P < 0.0001, by 2-way ANOVA Tukey’s multiple comparisons test. K, L UBE3B protein levels in Flag-VHL-expressing MDA-MB-231 (K) and T47D (L) cells with or without MG132 treatment. EV, empty vector. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38914543), licensed under a CC-BY license. Not internally tested by Novus Biologicals.