Ubiquitin-Propargylamide Protein

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R&D Systems Recombinant Proteins and Enzymes
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Ubiquitin-Propargylamide Protein Summary

Product Specifications

>95%, by HPLC
Add Recombinant Human Ubiquitin-Propargylamide to in vitro assays to inhibit deubiquitinating enzymes. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Propargylamide concentration of 0.5-5 μM.
Chemically Synthesized Ubiquitin protein
Predicted Molecular Mass
8.5 kDa

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a solution in Acetonitrile, Acetic Acid, deionized water.
Reconstitution Reconstitute at 2 mg/ml in DMSO.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Ubiquitin

Ubiquitin-Propargylamine (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s). When mixed together, the reactive site cysteine of deubiquitinases and the C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions. This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

  1. Ekkebus R., et al. (2013) J. Am. Chem. Soc 135: 2867-2870.  
  2. Sommer S., et al. (2013) Bioorg Med Chem. 21:2511-7.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citation for Ubiquitin-Propargylamide Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural basis for the dual catalytic activity of the Legionella pneumophila ovarian-tumor (OTU) domain deubiquitinase LotA
    Authors: J Luo, X Ruan, Z Huang, Z Li, L Ye, Y Wu, X Zhen, S Ouyang
    The Journal of Biological Chemistry, 2022;0(0):102414.
    Species: Bacteria - Legionella pneumophila
    Sample Types: Recombinant Protein
    Applications: Bioassay


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Ubiquitin-Propargylamide Protein
By Tao An on 06/01/2019
Application: In vitro bioactivity in cell culture
Reason for Rating: USP15 is well labeled with this probe.

293T cell lysates (40μg)were incubated with this probe for 15 min at 37℃。