Ubiquitin-Propargylamide Protein

R&D Systems | Catalog # U-214

R&D Systems
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Key Product Details

  • R&D Systems Synthetic-derived Ubiquitin-Propargylamide Protein (U-214)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Synthetic

Accession Number

P0CG47.1

Structure / Form

Modified

Conjugate

Propargylamide

Applications

Bioactivity
View all Ubiquitin Proteins and Enzymes »

Product Specifications

Source

Chemically Synthesized Ubiquitin protein
Met1 - Gly75

Purity

>95%, by HPLC.

Predicted Molecular Mass

8.5 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Propargylamide concentration of 0.5-5 μM.

Reviewed Applications

Read 1 review rated 4 using U-214 in the following applications:

Formulation, Preparation, and Storage

U-214
Formulation Lyophilized from a solution containing Acetonitrile and TFA.
Reconstitution Reconstitute at 2 mg/ml in DMSO.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin-Propargylamine (Ub-PA) is a potent, irreversible and specific inhibitor of C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs), and other deubiquitinases including the Otubains (OTU’s). When mixed together, the reactive site cysteine of deubiquitinases and the C-terminal alkyne functional group of Ub-PA rapidly form a covalent bond (vinyl thioether) that is resistant to denaturing conditions. This product is useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation.

References

  1. Ekkebus, R., et al. (2013) J. Am. Chem. Soc 135:2867.   
  2. Sommer, S., et al. (2013) Bioorg Med Chem. 21:2511.

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

UniProt

P0CG47.1

Additional Ubiquitin Products

Product Documents for Ubiquitin-Propargylamide Protein

Certificate of Analysis

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Product Specific Notices for Ubiquitin-Propargylamide Protein

For research use only

Citations for Ubiquitin-Propargylamide Protein

Customer Reviews for Ubiquitin-Propargylamide Protein (1)

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  • Ubiquitin-Propargylamide Protein
    Name: Tao An
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/01/2019
    293T cell lysates （40μg）were incubated with this probe for 15 min at 37℃。
    Ubiquitin-Propargylamide Protein U-214

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