UCH-L1/PGP9.5 Antibody (13C4)
Novus Biologicals | Catalog # NBP2-29420
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Rabbit, Sheep, Zebrafish
Cited:
Mouse
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 13C4
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Product Specifications
Immunogen
UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)
Localization
Cytoplasmic. Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33186)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for UCH-L1/PGP9.5 Antibody (13C4)
Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]
Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]
Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]
Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420]
Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Electropherogram image of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] -
qRT–PCR and western blot analysis. A–C The top bar graph presents the mRNA/protein levels (Uchl1/UCHL1, Tmprss13/TMPRSS13, and Cst3/CST3) in the diaphragm for the MV and control groups. The mRNA expression levels of three genes measured by quantitative real-time PCR were normalized with respect to beta -actin and are expressed as fold changes relative to the control levels. The protein expression levels were also normalized with respect to beta -actin. The bottom panel presents the western blotting results for UCHL1, TMPRSS13, CST3, and beta -ACTIN protein levels in the diaphragm for the MV and control groups. The data are presented as the mean +/- SD (n=6); “*" indicates P < 0.05 compared with the control group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36085166), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for UCH-L1/PGP9.5 Antibody (13C4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Simple Western
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Cerebellum and IMR-32 lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 31 kDa.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in h. Cerebellum and IMR-32 lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 31 kDa.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: UCH-L1/PGP9.5
A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells.
Long Name
Ubiquitin C-terminal Hydrolase L1
Alternate Names
PARK5, PGP9.5, UCHL1
Gene Symbol
UCHL1
UniProt
Additional UCH-L1/PGP9.5 Products
Product Documents for UCH-L1/PGP9.5 Antibody (13C4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UCH-L1/PGP9.5 Antibody (13C4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for UCH-L1/PGP9.5 Antibody (13C4)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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