Recombinant Mouse UCH-L3 His Protein

Novus Biologicals | Catalog # NBP2-52385

Novus Biologicals
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Key Product Details

Source

Baculovirus

Tag

His

Applications

Enzyme Activity, SDS-PAGE
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Product Specifications

Description

A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-230 of Mouse UCH-L3

Source: Baculovirus

Amino Acid Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse UCH-L3 His Protein

SDS-PAGE: Recombinant Mouse UCH-L3 His Protein [NBP2-52385]

SDS-PAGE: Recombinant Mouse UCH-L3 His Protein [NBP2-52385]

SDS-Page: UCH-L3 Recombinant Protein [NBP2-52385] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52385
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L3

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for Recombinant Mouse UCH-L3 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse UCH-L3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

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FAQs

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