Ulex Europaeus Lectin 1 Native Protein

Novus Biologicals | Catalog # NBP2-42700

Novus Biologicals
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Key Product Details

Applications

Bioactivity, SDS-PAGE
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Product Specifications

Description

A biologically active protein corresponding to Ulex Europaeus Lectin 1.

Purity

>50% SDS-PAGE

Predicted Molecular Mass

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Agglutination activity is expressed in ug/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 degrees C.

Application Notes

Ulex europaeus agglutinin has anti-H blood group specificity.

Protein / Peptide Type

Native Protein

Scientific Data Images for Ulex Europaeus Lectin 1 Native Protein

Product Image: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700] - Lectin from Ulex europaeus (gorse, furze) at a concentration of ~1mg solid/mL was serially diluted (2-fold, top to bottom of plate) and incubated with human erythrocytes type: O (4% v/v RBC) to calculate Minimum agglutination titer (MAT). Agglutination is defined as the inability of cells to form a tight button. PBS was used as blank.
SDS-PAGE: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700]

SDS-PAGE: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700]

SDS-Page: Ulex Europaeus Lectin 1 Native Protein [NBP2-42700] - Lectin from Ulex europaeus (gorse, furze) was assessed on SDS-PAGE.

Formulation, Preparation, and Storage

NBP2-42700
Formulation Lyophilized in buffer. Information about buffer components is properietary.
Preservative No Preservative
Concentration Lyoph
Reconstitution Reconstitute with PBS (pH 6.8) to a final concentration of 1mg/ml.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ulex Europaeus Lectin 1

Ulex europaeus agglutinin I, a lectin, binds to many glycoproteins and glycolipids containing alpha-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells.There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N'-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Alternate Names

Gorse or Furze Lectin, UEA-1; H(O) lectin 1; Anti H(O) lectin I

Additional Ulex Europaeus Lectin 1 Products

Product Documents for Ulex Europaeus Lectin 1 Native Protein

Certificate of Analysis

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Product Specific Notices for Ulex Europaeus Lectin 1 Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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