Q: I have a question about your Uroplakin III protein (#NBP1-72543). How could you measure Uroplakin III activity after purify? cause i need this protein later if activity is good.

A: The lab has informed me that because we haven't measured the activity of Uroplakin III, we regrettably don't have any information or useful advice.

Recombinant Human Uroplakin III His Protein

Novus Biologicals | Catalog # NBP1-72543

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Uroplakin III Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-207 of Human Uroplakin III

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Uroplakin III His Protein

SDS-PAGE: Recombinant Human Uroplakin III His Protein [NBP1-72543]

SDS-Page: Recombinant Human Uroplakin III His Protein [NBP1-72543] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-72543

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 150 mM NaCl
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Uroplakin III

UPK3A belongs to the uroplakin-3 family. Uroplakins (UP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (UPII, UPIIIa, and UPIIIb) or four times (UPIa and UPIb; both belonging to the 'tetraspanin' family). All have large luminal/extracellular domains, but only UPIIIa and UPIIIb have significant cytoplasmic portions in their C-termini. UPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia Recombinant human UPK3A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternate Names

MGC119178, UP3a, UPIII, UPIIIA, UPK3, UPK3A, uroplakin 3, uroplakin 3A, Uroplakin III, uroplakin3a, uroplakin-3a

Gene Symbol

UPK3A

Additional Uroplakin III Products

Product Documents for Recombinant Human Uroplakin III His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Uroplakin III His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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