USP16 Antibody

Novus Biologicals | Catalog # NB100-81639

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human ubiquitin specific peptidase 16 using the numbering given in entry NP_006438.1 (GeneID 10600).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP16 Antibody

Immunoprecipitation: USP16 Antibody [NB100-81639]

Immunoprecipitation: USP16 Antibody [NB100-81639]

Immunoprecipitation: USP16 Antibody [NB100-81639] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody used for IP at 6 ug per reaction. USP16 was also immunoprecipitated by rabbit anti-USP16 antibody. For blotting immunoprecipitated USP16 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for USP16 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP16

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 16 (USP16) is a member of this family. USP16 activity is cell cycle regulated via phosphorylation and participates in cell cycle control by influencing mitotic chromosome condensation and histone 2A (H2A) deubiquitination.

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Entrez Gene IDs

10600 (Human)

Gene Symbol

USP16

UniProt

Additional USP16 Products

Product Documents for USP16 Antibody

Certificate of Analysis

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Product Specific Notices for USP16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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