VAChT/SLC18A3 Antibody

Novus Biologicals | Catalog # NB100-91348

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VAChT/SLC18A3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human VAChT/SLC18A3 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Reactivity Notes

Marmoset

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAChT/SLC18A3 Antibody

Western Blot: VAChT/SLC18A3 Antibody [NB100-91348]

Western Blot: VAChT/SLC18A3 Antibody [NB100-91348]

Western Blot: VAChT/SLC18A3 Antibody [NB100-91348] - Rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - Rat spinal cord. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.
Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348]

Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.

Applications for VAChT/SLC18A3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAChT/SLC18A3

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Entrez Gene IDs

6572 (Human); 20508 (Mouse); 60422 (Rat)

Gene Symbol

SLC18A3

UniProt

Q16572 (Human)

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VAChT/SLC18A3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAChT/SLC18A3 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VAChT/SLC18A3 Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB100-91348?

    A: The epitope for NB100-91348 is between aa region 450-505 of human VACHT raised in rabbit (whole antiserum).

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB100-91348?

    A: The epitope for NB100-91348 is between aa region 450-505 of human VACHT raised in rabbit (whole antiserum).

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