VAChT/SLC18A3 Antibody

Novus Biologicals | Catalog # NB110-74764

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VAChT/SLC18A3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 450-510 of human VAChT/SLC18A3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Localization

Plasma membrane

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for VAChT/SLC18A3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000
Application Notes
IHC-FR reactivity reported in (PMID:24400086).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera with PBS

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAChT/SLC18A3

FUNCTION: Involved in acetylcholine transport into synaptic vesicles.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle. SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Entrez Gene IDs

6572 (Human); 20508 (Mouse); 60422 (Rat)

Gene Symbol

SLC18A3

UniProt

Q16572 (Human), O35304 (Mouse), Q62666 (Rat)

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VAChT/SLC18A3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAChT/SLC18A3 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VAChT/SLC18A3 Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB110-74764?

    A: The epitope for NB110-74764 is between aa region 450-505 of human VACHT raised in goat (whole antiserum).

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB110-74764?

    A: The epitope for NB110-74764 is between aa region 450-505 of human VACHT raised in goat (whole antiserum).

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