Detection of HHV8‑ORF74 in HEK293 Human Cell Line Transfected with Viral HHV8-ORF74 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with viral HHV8-ORF74 and eGFP was stained with either (A) Mouse Anti-Viral HHV8‑ORF74 APC‑conjugated Monoclonal Antibody (Catalog # FAB5264A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The herpes family represents about 130-140 double-stranded DNA viruses that infect almost all phyla in the animal kingdom. HHV8 (Human Herpes Virus-8) is one of eight herpes viruses known to infect humans. These eight viruses are categorized into three classes: alpha (HHV1-3 that infect neurons), beta (HHV5-7) and gamma (HHV4 and 8 that lie latent in B cells). HHV8 contains about 100 genes/ORFs, including viral homologues for MIP (vMIP), Bcl-2, IL-6, NCAM and IL-8 R2. HHV8-ORF74 is a homolog of IL-8 R2, and when expressed shows a 44-46 kDa G-protein coupled receptor that belongs to the rhodopsin/ beta -adrenergic family of GCPRs. Unlike IL-8 R2, HHV8-ORF74 is constitutively active. Its activity is regulated by multiple chemokines that can either upregulate basal activity (IL-8; GRO-alpha ), downregulate basal activity (IP-10; vMIP-II; SFD-1) or leave activity untouched (NAP-2; ENA-78), acting instead as a blocking factor. HHV8-ORF74 activation on infected endothelial cells appears to drive them into a spindle-shape, characteristic of that seen in Kaposi's sarcoma, and induces the expression of multiple proangiogenic molecules such as IL-6, IL-8, ICAM-1, VCAM-1 and VEGF plus VEGF R2. These effects appear to be necessary, but not necessarily sufficient, for Kaposi's sarcoma development. The ORF74 GPCR shares 29% and 27% amino acid sequence identity with human CXCR2 and CXCR1, respectively.
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