VisUCyte HRP Polymer

VisUCyte™ HRP Polymer - Cleaner, Faster IHC Results

VisUCyte™ HRP Polymer is a Biotin-free detection reagent that overcomes problems associated with Avidin-Biotin detection chemistry, such as the extra quenching steps required to inhibit endogenous Biotin and Avidin staining. R&D Systems offers secondary antibodies that are conjugated to the VisUCyte™ HRP Polymer and complete staining kits that contain VisUCyte™ HRP Polymer-conjugated secondary antibodies, the DAB chromogen, buffers, and blocking reagent.

 

VisUCyte™ HRP Polymer Benefits

  • Improved Chromogenic IHC Staining
  • More Sensitive (Use 2–3 Times Less Primary Antibody)
  • Reduced Background
  • Faster (Less Blocking Steps/Incubations)
  • Prediluted, Ready-to-Use
  • Compatible with Most Fixation Methods
 

Compare IHC Staining Using the VisUCyte™ HRP Polymer versus Avidin-Biotin

 
GIT1 detection using the VisUCyte HRP Polymer

GIT1 Detection in Human Brain Using the VisUCyte HRP Polymer. GIT1 was detected in immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) using a Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog #AF8508), followed by a Goat Anti-Rabbit IgG VisUCyte HRP Polymer Secondary Antibody (Catalog #VC003). The tissue was stained with DAB (brown) and counterstained with hematoxylin (blue).
GIT1 detection using ABC method

GIT1 Detection in Human Brain Using the ABC Method. GIT1 was detected in immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) using a Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog #AF8508), followed by a Biotin-conjugated anti-rabbit secondary antibody. The tissue was stained using the ABC Method (brown) and counterstained with hematoxylin (blue).
Secondary Antibody Control for the VisUCyte™ HRP Polymer.

Secondary Antibody Control for the VisUCyte HRP Polymer. Immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) were incubated with a Goat Anti-Rabbit IgG VisUCyte HRP Polymer Secondary Antibody (Catalog # VC003), followed by DAB. The tissue was counterstained with hematoxylin (blue). The Rabbit Anti-Human GIT1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF8508) was omitted from the staining procedure.
 

View Our Protocol for Using the VisUCyte™ HRP Polymer

 

Learn More about Our IHC/ICC-Validated Antibodies