Rabbit IgG VisUCyte HRP Polymer Antibody

  
  • Species Reactivity
    Rabbit
  • Specificity
    Detects heavy ( gamma ) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rabbit IgG
  • Formulation
    Supplied as a solution in PBS with preservative.
  • Label
    HRP Polymer
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Detectionof SKG in Human Kidney.  SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 21  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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