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- Species ReactivityRabbit
- SpecificityDetects heavy ( gamma ) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenRabbit IgG
- FormulationSupplied as a solution in PBS with preservative.
- LabelHRP Polymer
Applications
-
Recommended
ConcentrationSample
-
ImmunohistochemistryPrediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
|
Immunohistochemistry
|
Detectionof SKG in Human Kidney. SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color). |
Preparation and Storage
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
- Stability & Storage6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
VisUCyte™ HRP Polymer is a
biotin-free detection reagent which overcomes problems of avidin-biotin
detection chemistry, such as endogenous biotin staining and the need to do
additional quenching of endogenous biotin and avidin present in
some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells
and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may
be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
- Long Name:Immunoglobulin G
- Alternate Names:IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Secondary Antibodies
Description
Application
Cat#
Citations
Images
Rabbit IgG HRP-conjugated Antibody
WB HAF008 21View Sizes & Prices
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody
Flow, IHC, ICC NL004 10View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody
Flow, IHC, ICC NL005 2View Sizes & Prices
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Rabbit IgG APC-conjugated Antibody
Flow F0111 1View Sizes & Prices
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Rabbit IgG PE-conjugated Antibody
Flow F0110 2View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Rabbit IgG Fluorescein-conjugated Antibody
Flow F0112 5View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody
Flow, IHC, ICC NL006 4View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Goat Anti-Rabbit IgG Biotinylated Antibody
WB BAF008 4View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody
IHC VC002View Sizes & Prices
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Rabbit IgG Antibody
WB AF008 1View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Donkey Anti-Rabbit IgG (H+L) Antibody
D-301-C-ABS2View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
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Calculators
Reconstitution Calculator
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
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