Detects heavy ( gamma ) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Polyclonal Goat IgG
Supplied as a solution in PBS with preservative.
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detectionof SKG in Human Kidney. SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.
VisUCyte™ HRP Polymer is a
biotin-free detection reagent which overcomes problems of avidin-biotin
detection chemistry, such as endogenous biotin staining and the need to do
additional quenching of endogenous biotin and avidin present in
some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells
and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may
be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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