Rabbit IgG VisUCyte HRP Polymer Antibody

VC003 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
VC003-025
VC003-050
VC003-125
VC003-250

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Rabbit IgG VisUCyte HRP Polymer Antibody Summary

Species Reactivity

Rabbit

Specificity

Detects heavy ( gamma ) chains on rabbit IgG and light chains on all rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rabbit serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Rabbit IgG

Formulation

Supplied as a solution in PBS with preservative.

Label

HRP Polymer

Applications

Recommended Concentration

Sample

Immunohistochemistry

Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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Detection of SKG in Human Kidney. SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name

Immunoglobulin G

Alternate Names

IgG