Rabbit IgG VisUCyte HRP Polymer Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detectionof SKG in Human Kidney. SKG was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed byincubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003). Tissue was stained with DAB (brown color) andcounterstained with hematoxylin (blue color).
Preparation and Storage
VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.
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